The separations are greatly improved, however, through the use of high-resolution columns and the column retentions times are much reduced. The narrow and relatively long columns are packed with a noncompressible matrix of fine glass or plastic beads coated with a thin layer of the stationary phase. Alternatively, matrix may consist of silica whose available […]

In high-performance liquid chromatography (HPLC), a liquid solvent containing a mixture of molecules to be identified is passed through a column densely packed with a small-diameter insoluble bead-like resin. In column chromatography, the smaller and more tightly packed the resin beads, the greater the resolution of the separation technique. In HPLC, the resin is so […]

Immunochemical procedures provide protein assay techniques of high sensitivity and discrimination. These methods employ antibodies, proteins produced by an animal’s immune system in response to the introduction of a foreign protein and which specifically bind to this foreign protein.

The technique exploits the specificity of reaction between an antigen and antibody and molecular sieving of the gel in which this reaction is taking place for analysis of components of a given sample. The technique was developed by Grabar and Williams and is actually a modification of Ouchterlony double diffusion technique. To understand the technique […]

A major portion of most biochemical investigations involves the purification of the materials under consideration because these substances must be relatively free of contaminants if they are to be properly characterized. This is often a formidable task because a typical cell contains thousands of different substances, many of which closely resemble other cellular constituents in […]